This book explains the advancements of plant biotechnology and advanced molecular biology and explores the details of influential tools that complement conventional breeding and accelerate the development of plants resilient to adverse agroclimatic conditions and biofortified plants. Plant biotechnology from the basic sciences to current applications, such as pathway engineering, precursor feeding, transformation, elicitation with biotic and abiotic elicitors, and scaling up in bioreactors, have been included in these chapters to improve the production of secondary metabolites from different medicinal plants. It also highlights important factors often overlooked by methodologies used to develop plants tolerance against biotic and abiotic stresses and in developing special foods, bio-chemicals, and pharmaceuticals. This book is valuable for researchers or students working on biosciences. It is also an updated and advanced reference material for the agriculture and pharmaceutical industries.