This book constitutes the refereed proceedings of the 8th International Workshop on Ophthalmic Medical Image Analysis, OMIA 2021, held in conjunction with the 24th International Conference on Medical Imaging and Computer-Assisted Intervention, MICCAI 2021, in Strasbourg, France, in September 2021.* The 20 papers presented at OMIA 2021 were carefully reviewed and selected from 31 submissions. The papers cover various topics in the field of ophthalmic medical image analysis and challenges in terms of reliability and validation, number and type of conditions considered, multi-modal analysis (e.g., fundus, optical coherence tomography, scanning laser ophthalmoscopy), novel imaging technologies, and the effective transfer of advanced computer vision and machine learning technologies. *The workshop was held virtually.