This compelling book presents remarkable contributions from esteemed speakers who participated in the 28th International Conference on Difference Equations and Applications, ICDEA 2023, hosted at Phitsanulok, Thailand, in collaboration with the International Society of Difference Equations (ISDE) from July 17 to 21, 2023. Embark on an enriching journey through the realms of difference equations and discrete dynamical systems, with practical applications spanning economics, engineering, biology, and related sciences. Immerse yourself in cutting-edge research as you explore new and significant breakthroughs within the fields of difference equations and discrete dynamical systems. This comprehensive compilation spans diverse scientific disciplines, demonstrating the profound impact of these theories and methodologies in practical and tangible settings. From economic modeling to engineering optimization, from biological systems to interdisciplinary applications, this book exemplifies the versatility and relevance of difference equations and discrete dynamical systems. Designed to resonate with a wide audience, including Ph.D. students, researchers, educators, and practitioners, it goes beyond mere dissemination of recent results. It actively fosters the advancement of knowledge, serving as a catalyst for further explorations and innovations across the spectrum of sciences.