This book constitutes the refereed proceedings of the 12th International Conference on Language and Automata Theory and Applications, LATA 2018, held in Ramat Gan, Israel, in April 2018. The 20 revised full papers presented together with 3 invited papers were carefully reviewed and selected from 58 submissions. The papers cover fields like algebraic language theory, algorithms for semi-structured data mining, algorithms on automata and words, automata and logic, automata for system analysis and programme verification, automata networks, automatic structures, codes, combinatorics on words, computational complexity, concurrency and Petri nets, data and image compression, descriptional complexity, foundations of finite state technology, foundations of XML, grammars (Chomsky hierarchy, contextual, unification, categorial, etc.), grammatical inference and algorithmic learning, graphs and graph transformation, language varieties and semigroups, language-based cryptography, mathematical and logical foundations of programming methodologies, parallel and regulated rewriting, parsing, patterns, power series, string processing algorithms, symbolic dynamics, term rewriting, transducers, trees, tree languages and tree automata, and weighted automata.