This book studies the design optimization, state estimation, and advanced control methods for cyber-physical vehicle systems (CPVS) and their applications in real-world automotive systems. First, in Chapter 1, key challenges and state-of-the-art of vehicle design and control in the context of cyber-physical systems are introduced. In Chapter 2, a cyber-physical system (CPS) based framework is proposed for high-level co-design optimization of the plant and controller parameters for CPVS, in view of vehicles dynamic performance, drivability, and energy along with different driving styles. System description, requirements, constraints, optimization objectives, and methodology are investigated. In Chapter 3, an Artificial-Neural-Network-based estimation method is studied for accurate state estimation of CPVS. In Chapter 4, a high-precision controller is designed for a safety-critical CPVS. The detailed control synthesis and experimental validation are presented. The application results presented throughout the book validate the feasibility and effectiveness of the proposed theoretical methods of design, estimation, control, and optimization for cyber-physical vehicle systems.